Journal: Cells
Article Title: Extracellular Vesicles Derived from VEGF mRNA-Engineered Mesenchymal Stem Cells Promote Endothelial Cell Survival
doi: 10.3390/cells15080717
Figure Lengend Snippet: Angiogenesis induced by VEGF secreted from VEGF-MSCs. Human umbilical vein endothelial cells labelled by CytoLight Green (GFP-HUVECs) were used for the development of angiogenic networks, and the length of networks was determined by the IncuCyte live-cell analysis system. ( A ) VEGF effectively induced the formation of vascular networks, which was able to be inhibited by suramin, a VEGF signaling inhibitor. Data shown as mean (SD) ( n = 6). ( B ) A concentration-dependent effect was shown in the angiogenesis assay using CCM-VEGF with different VEGF concentrations (1, 2, 4, and 8 ng/mL). Blank control, without any VEGF supplement added; suramin group, 4 ng/mL VEGF and 100 µM suramin added. Data shown as mean (SD) ( n = 4); ns, not significant; **** p < 0.05.
Article Snippet: IncuCyte Angiogenesis 96-well PrimeKit (Essen Bioscience, Ann Arbor, MI, USA, catalog# 4452) and IncuCyte Angiogenesis PrimeKit VEGF/Suramin Supplement Kit (Essen Bioscience, catalog# 4437) were used as per the manufacturer’s protocols with some modifications.
Techniques: Cell Analysis, Concentration Assay, Angiogenesis Assay, Control